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NOD/ShiLtJ-Gt(ROSA)26Sorem1(CAG-cas9*, -EGFP)Dvs/DvsJ
Cat. No.: CEMM-07250645
Common Name: Rosa26CAG-LSL-Cas9-P2A-EGFP (RCL-Cas9-EGFP)
The Rosa26CAG-LSL-Cas9-P2A-EGFP knock-in allele (RCL-Cas9-EGFP) allows Cre recombinase-dependent expression of CRISPR associated protein 9 (cas9) endonuclease and EGFP. When these mice are used in combination with guide sequences and a Cre recombinase source, they allow editing of single or multiple mouse genes both in vivo and ex vivo.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sorem1(CAG-cas9*,-EGFP)Dvs
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| Allele Name |
endonuclease-mediated mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Endonuclease
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
cas9, CRISPR associated protein 9; GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
Expression of cas9 and EGFP is obtained in tissues expressing Cre recombinase driven by a desired promoter.
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| Strain of Origin |
NOD/ShiLtJ
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| Molecular Note |
Sequences include CMV enhancer/chicken beta-actin promoter (CAG), a loxP-STOP(3xSV40 polyA)-loxP cassette (LSL), a 3xFLAG sequence, a mammalian codon-optimized Cas9 gene (derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]), a P2A ribosomal skip cleavage peptide sequence, an enhanced green fluorescent protein gene (EGFP), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) and a bovine growth hormone polyA sequence (BGHpA). To facilitate insertion into the Gt(ROSA)26Sor locus on chromosome 6 via homology directed repair (HDR), the LSL-Cas9-P2A-EGFP sequences were co-injected with a large piece of Gt(ROSA)26Sor locus homologous DNA.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
NOD/ShiLtJ
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| Breeding Considerations |
Mice homozygous for the Rosa26CAG-LSL-Cas9-P2A-EGFP knock-in allele are viable and fertile. Mice on the NOD/ShiLtJ inbred background develop spontaneous autoimmune type 1 diabetes.When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to NOD/ShiLtJ inbred mice. Alternatively, homozygous mice may be bred together.
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For Research Use Only.
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