D2.Cg-Tg(Thy1-Brainbow1.0)LLich/SjJ

Tg(Thy1-Brainbow1.0)LLich

transgene insertion L

Reporter

Tg(Thy1-Brainbow1.0)LLich

transgene insertion L

UN

YFP, Yellow Fluorescent Protein, jellyfish; CFP, Cyan Fluorescent Protein, jellyfish; RFP, Red Fluorescent Protein, coral

3761287

tdimer2(12) (RFP), is expressed in peripheral and central neurons. When bred to Cre recombinase expressing mice, there is one of three expression outcomes for each transgene copy in each cell of the Cre expressing tissue(s): tdimer2(12) (RFP) (no recombination), mYFP, or mCerulean (CFP). Integration of multiple tandem transgene copies allows for labeling of individual neurons and glia (specifically hippocampal neuron cell bodies) with approximately 166 distinguishable color variations in Cre recombined cells.

C57BL/6 x CBA

The Thy1-Brainbow 1.0 transgene was designed with the mouse Thy1 regulatory elements surrounding three fluorescent protein (red (RFP), cyan (CFP), or enhanced yellow (EYFP)) sequences uniquely flanked with pairs of incompatible Lox sites. Specifically, this Brainbow 1.0 coding region contained (from 5' to 3') a Lox2272 site, LoxP site, dTomato RFP (with polyA sequence), Lox2272 site, mCerulean CFP (with polyA sequence), LoxP site, monomeric EYFP (mYFPA206K; with 5' FRT site and polyA sequence).The dTomato RFP is an optimized DsRed fluorescent protein variant with a combination of amino acid substitutions (V22M/Q66M/V105L/F124M) designed to improve spectral properties. The monomeric Cerulean (mCerulean) is a variant of ECFP (ECFPS72A/Y145A/H148D/A206K) with amino acid substitutions designed to improve spectral properties and prevent dimerization. The monomeric EYFP (mYFPA206K) has an amino acid substitution replacing a hydrophobic region with a positively charged residue designed to prevent dimerization.

DBA/2J

When maintaining a live colony, homozygous mice may be bred together.

Homozygote x Homozygote