Online Inquiry
D2.Cg-Tg(Plp1-cre/ERT)3Pop/SjJ
Cat. No.: CEMM-07250547
These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Plp1, proteolipid protein (myelin) 1 promoter. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration allows for ablation of predetermined genes in oligodendrocytes and Schwann cells, and will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(Plp1-cre/ERT)3Pop
show more
close
|
| Allele Name |
transgene insertion 3
show more
close
|
| Allele Attributes |
Recombinase-expressing; Inducible
show more
close
|
| Gene Symbol |
Tg(Plp1-cre/ERT)3Pop
show more
close
|
| Gene Name |
transgene insertion 3
show more
close
|
| Chromosome |
UN
show more
close
|
| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1; ESR1, estrogen receptor 1, human
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
Tamoxifen inducible Cre recombinase protein fused to mutant form of the mouse estrogen receptor ligand binding domain; expression in oligodendrocytes and Schwann cells
show more
close
|
| Strain of Origin |
(C57BL/6 x DBA/2)F1
show more
close
|
| Molecular Note |
The transgenic construct encodes a tamoxifen-inducible fusion protein consisting of Cre recombinase and a mutated human estrogen receptor ligand-binding domain cloned next to a Plp promoter. The 2.5 kb Plp regulatory region includes 5' flanking DNA, exon 1, and intron 1. Transgene expression was confirmed by RT-PCR and Western blot analysis. The mouse Plp promoter was used to drive transgene expression in myelinating cells.
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
DBA/2J Noncarrier
show more
close
|
| Breeding Considerations |
Hemizygotes are viable and fertile.
show more
close
|
| Breeding Strategy |
Noncarrier x Hemizygote; Hemizygote x Noncarrier
show more
close
|
For Research Use Only.
Related Products