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B6.FVB-Tg(Slc32a1-cre)2.1Hzo/FrkJ
Cat. No.: CEMM-07250354
Common Name: Viaat-cre
Mice hemizygous for the BAC Viaat-cre transgene are viable and fertile, with solute carrier family 32 (GABA vesicular transporter), member 1 (Slc32a1 or Viaat) promoter/enhancer regions driving expression of cre-recombinase. These mice may be useful for studying GABAergic neuronal function.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(Slc32a1-cre)2.1Hzo
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| Allele Name |
transgene insertion 2.1
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Tg(Slc32a1-cre)2.1Hzo
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| Gene Name |
transgene insertion 2.1
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| Chromosome |
UN
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
When mice carrying this transgene are bred with mice containing loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequences in GABAergic neurons of the offspring.
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| Strain of Origin |
FVB/N
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| Molecular Note |
BAC RP23-392P11 containing the Slc32a1 (Viaat) locus was modified by homologous recombination by inserting a Cre-recombinase sequence followed by a stop codon and a polyadenylation sequence downstream of the start codon of the Slc32a1 (or Viaat) gene. After linearization, the BAC only contained Viaat and the truncated coding sequence of the actin-related protein 5 homolog (Actr5) gene. This modified 109 kb BAC was microinjected into fertilized FVB/N oocytes. Transgenic animals have similar expression levels of Slc32a1 and Actr5 to wild type mice. Founder line 2.1 was analyzed and propagated.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or C57BL/6J inbred mice. For Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding BAC Viaat-cre females to floxed males. See Detailed Description for more details and suggestions.
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For Research Use Only.
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