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B6.Cg-Snap25tm1.1Hze/J
Cat. No.: CEMM-07250464
Common Name: Snap25-LSL-2A-EGFP-D
The Snap25-LSL-2A-EGFP-D (also called Snap25-LSL-2A-EGFP-Δ or Snap25-LSL-2A-EGFP-Δneo/hygro) conditional allele is designed with a loxP-flanked STOP cassette and enhanced green fluorescent protein (EGFP) downstream of the synaptosomal-associated protein 25 (Snap25) translational STOP codon. Following Cre recombinase removal of the floxed STOP cassette, EGFP fluorescence is expected in cells/tissues where the expression patterns of Snap25 and Cre recombinase overlap. Snap25 is a t-SNARE protein. These mice may be useful as a Cre-lox tool for fluorescent visualization of synaptic vesicle/plasma membrane fusion.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Snap25tm1.1Hze
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| Allele Name |
targeted mutation 1.1
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| Allele Attributes |
Reporter; Null/Knockout
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| Gene Symbol |
Snap25
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| Gene Name |
synaptosomal-associated protein 25
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| Chromosome |
2
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| Expressed Genes |
GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
EGFP fluorescence is expected in diverse neuronal subpopulations.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The targeting vector contained, from 5' to 3', a partial Snap25 sequence spanning intron 6 through intron 7, an frt3 site within Snap25 intron 7, a partial Snap25 exon 8 sequence up to (but not including) the endogenous stop codon, a loxP-flanked STOP cassette that is in-frame with theupstream Snap25 exon 8 sequence (contains translational stop codons in all three reading frames and the PGK polyA sequence), an in-frame F2A peptide cleavage signal, an in frame EGFP gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA signal, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 late polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequences (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL).
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J Wild-type from the colony
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| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony. The donating investigator has not attempted to generate homozygous mice to date.
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For Research Use Only.
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