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B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J
Cat. No.: CEMM-07250151
Common Name: Wfs1-Tg3-CreERT2
These Wfs1-Tg3-CreERT2 mice express a tamoxifen-inducible Cre recombinase under the control of the mouse Wfs1 (Wolfram syndrome 1 homolog (human)) promoter/enhancer regions, and may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues (including cortex, hippocampus, striatum, thalamus and cerebellum).
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(Wfs1-cre/ERT2)3Aibs
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| Allele Name |
transgene insertion 3
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| Allele Attributes |
Recombinase-expressing; Inducible
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| Gene Symbol |
Tg(Wfs1-cre/ERT2)3Aibs
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| Gene Name |
transgene insertion 3
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| Chromosome |
UN
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1; ESR1, estrogen receptor 1, human
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| MGI Accession ID | show more close |
| Site of Expression |
Hemizygous Wfs1-Tg3-CreERT2 mice are viable and fertile. As the Cre-ERT2 fusion gene is under control of the Wfs1 promoter/enhancer regions within the BAC transgene, Cre activity is directed to cortex, hippocampus, striatum, thalamus and cerebellum only following taxomifen administration.
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| Strain of Origin |
(C57BL/6 x C3H)F1 x C57BL/6
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| Molecular Note |
The 196 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-405O19, containing the entire Wsf1 locus (and other genes), was modified by targeting a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), SV40 polyA signal, and FRT flanked neomycin cassette to the ATG start site of the Wsf1 locus. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. Cre activity is directed to the cortex, hippocampus, striatum, thalamus, and cerebellum only following tamoxifen administration.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6C3F1/J C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, transgenic carrier mice may be bred together, to wildtype (non-carrier) siblings, or to C57BL/6J inbred mice.
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For Research Use Only.
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