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STOCK Gt(ROSA)26Sortm1.1(CAG-HL9-MG*V70A, -mBFP, -SNCA, -mTFP1, -SNCA*A30P, -mKO2, -SNCA*A53T)Rali Tg(Vil1-cre/ERT2)23Syr/J

Cat. No.: CEMM-07251120

Common Name: SNCAbow; Vil‐CreERT2
These SNCAbow;Vil-CreERT2 mice contain α‐synuclein-Crainbow (SNCAbow) reporter and a transgene with tamoxifen-inducible Cre recombinase expression in intestinal epithelium (Vil-CreERT2), making this strain useful for studying the role of α‐synuclein expression in gut mucosal cells in early stages of Parkinson's and related neurodegenerative diseases. α‐synuclein-Crainbow (SNCAbow) has a CAG promoter followed by three pairs of orthogonal lox sites (LoxN, Lox2272, and LoxP), and the SNCAbow construct, all targeted into the Gt(ROSA)26Sor locus. SNCAbow contains four fluorescent proteins, three of which are associated with three human α-synuclein protein sequences (SNCAWT, SNCAA30P, or SNCAA53T), and functions as a Cre recombinase-inducible reporter of either a chemically inducible near‐infrared fluorogen‐activating peptide (FAPMars1) or one of the three nuclear localized fluorescent proteins. This reporter allele is available on its own as. The Vil-CreERT2 transgene is available on its own as.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Gt(ROSA)26Sortm1.1(CAG-HL9-MG*V70A, -mBFP, -SNCA, -mTFP1, -SNCA*A30P, -mKO2, -SNCA*A53T)Rali
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Allele Name
targeted mutation 1.1
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Allele Attributes
Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence; Humanized sequence; Epitope tag
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Gene Symbol
Gt(ROSA)26Sor
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Gene Name
gene trap ROSA 26
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Chromosome
6
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MGI Accession ID
7332841
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Strain of Origin
(129S6/SvEvTac x C57BL/6NCrl)F1
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Molecular Note
A targeting vector contains (from 5' to 3') a strong CAG (CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid) promoter, three pairs of orthogonal lox sites (LoxN, Lox2272, and LoxP), and the SNCAbow construct (described in greater detail below), followed by an FRT flanked neomycin resistance (neo) cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The alpha‐synuclein-Crainbow construct contains three pairs of orthogonal lox sites (LoxN, Lox2272, and LoxP) that separate four cassettes, three of which are associated with a corresponding alpha-synuclein gene sequence. The first cassette expresses a chemically inducible near‐infrared fluorogen‐activating peptide (referred to as FAPMars1). Mars1 is a V70A mutation of the human single-chain antibody (scFv)-based fluorogen isolated from HL9-MG (human library 9 malachite green). The next three cassettes encode a unique nuclear localized fluorescent protein (TagBFP:blue, mTFP1:turquoise, or mKO2:orange) and a corresponding human alpha-synuclein protein sequence (SNCA(WT), SNCA(A30P), or SNCA(A53T), respectively) each separated by a P2A peptide to mediate ribosomal skipping. Each alpha-synuclein sequence contains a C-terminal epitope tag: SNCA(WT) tagged with V5, SNCA(A30P) tagged with 3XHA, or SNCA(A53T) tagged with Myc. To ensure that only one fluorescent protein is expressed at any one time, an SV40 polyadenylation signal was inserted downstream of each open reading frame. Upon Cre activation, the lox sites recombine to Create three mutually exclusive excision possibilities with the fluorescent protein that is placed first after the CAG promoter being expressed. FLP-mediated recombination removed the neomycin cassette.
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HUSBANDRY
Suggested Controls
129S
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Breeding Considerations
Mice homozygous for the SNCAbow allele are viable and fertile while the viability and fertility of mice homozygous for the Vil-creERT2 transgene is unknown. As such, we maintain our live colony by breeding mice homozygous for the SNCAbow allele and hemizygous for Vil-creERT2 to mice wildtype at the Vil-creERT2 locus.
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For Research Use Only.
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