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B6;129S4-Gt(ROSA)26Sortm3(CAG-tdTomato, -EGFP*)Zjh/J
Cat. No.: CEMM-07250678
Common Name: IS reporter
These IS reporter mice harbor a targeted mutation of the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette and a frt-flanked tdTomato sequence preventing transcription of a CAG promoter-driven enhanced green fluorescent protein (EGFP). tdTomato is expressed following Cre-mediated recombination. EGFP is only expressed following Cre-mediated and Flp-mediated recombination. This strain may be useful as an intersectional reporter for cre and FLP expression, or as a subtraction reporter for cre expression alone.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm3(CAG-tdTomato,-EGFP*)Zjh
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| Allele Name |
targeted mutation 3
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
RFP, Red Fluorescent Protein, coral; GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
tdTomato is expressed following Cre-mediated recombination. EGFP is only expressed following Cre-mediated and Flp-mediated recombination.
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| Strain of Origin |
(C57BL/6 x 129S4/SvJae)F1
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| Molecular Note |
The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, an FRT flanked tdTomato sequence (tdTomato is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), three copies of green fluorescent protein, and a Neo-polyA cassette. The three GFP sequences include emerald, TagGFP (Aequorea macrodactyla GFP), and hrGFP (Humanized Renilla reniformis GFP) variants. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
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| HUSBANDRY | |
|---|---|
| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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