B6;129S-Tg(CAG-Bgeo, -MYC)1Saa/J

Cat. No.: CEMM-07250658

Common Name: Z-MYC

This strain is undergoing genetic quality control evaluation and is currently unavailable.Z-Myc transgenic mice conditionally express human c-Myc. Widespread lacZ expression is observed except in those tissues where Cre recombination has resulted in the human c-Myc to be expressed instead. These mice allow inducible c-Myc overexpression in specific tissues, and are useful in studying cancer.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(CAG-Bgeo,-MYC)1Saa show more close
Allele Name	transgene insertion 1 show more close
Allele Attributes	Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence; Humanized sequence show more close
Gene Symbol	Tg(CAG-Bgeo,-MYC)1Saa show more close
Gene Name	transgene insertion 1 show more close
Chromosome	UN show more close
Expressed Genes	Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli; MYC, MYC proto-oncogene, bHLH transcription factor, human show more close
MGI Accession ID	5695550 show more close
Site of Expression	ß-galactosidase (LacZ) expression is widespread until combination with Cre recombinase When human c-Myc is then expressed in Cre-expressing tissues (with continued LacZ expression in other tissues). show more close
Strain of Origin	129S/SvEv show more close
Molecular Note	This transgene contains from 5' to 3', the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, a loxP-flanked STOP cassette (beta-galactosidase/neomycin resistance cassette [beta-geo] with three copies of the SV40 polyA termination signal) and the 1.8 kbp human myelocytomatosis oncogene cDNA sequence (MYC; including exons 2 and 3 and polyA site). The 10.8 kbp transgene was linearized and transfected into 129S/SvEv-derived embryonic stem (ES) cells. An ES cell clone with a single-copy transgene integration was selected for injection into blastocysts. Constitutive beta-galactosidase (lacZ) expression is directed to multiple tissue types. Removal of the STOP cassette by Cre-mediated recombination results in removal of lacZ and expression of MYC. show more close

HUSBANDRY
Suggested Controls	Noncarrier show more close
Breeding Considerations	When maintaining a live colony, transgenic mice may be bred with wildtype (non-carrier) mice from the colony or with C57BL/6J inbred mice. it has not been attempted to make this strain homozygous. show more close
Breeding Strategy	Hemizygote x Noncarrier; Noncarrier x Hemizygote show more close

For Research Use Only.