B6.129(Cg)-Arc^{tm1.1(cre/ERT2)Luo}/J

Arc^{tm1.1(cre/ERT2)Luo}

targeted mutation 1.1

Recombinase-expressing; Inducible

Arc

activity regulated cytoskeletal-associated protein

15

cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene

5488904

Following Tamoxifen induction, Cre recombinase activity is observed in active populations of neurons in the brain in a pattern consistent with endogenous Arc expression.

(129X1/SvJ x 129S1/Sv)F1-Kitl+

A targeting vector was designed to insert a Cre/ERT2-SV40 polyA cassette followed by an FRT5-flanked pSV40-NeoR-pA cassette into the the translational start site of the activity regulated cytoskeletal-associated protein gene (Arc). The targeting event displaced the introns and coding regions and replaced the endogenous 3' UTRs (which contribute to mRNA destabilization and to Arc mRNA dendritic trafficking) with an exogenous SV40 polyadenylation signal (to promote high-level expression). All sequences 5' to the translational start site are retained. The resulting chimeric animals were bred to germline-active GFP-FlpO transgenic mice to delete the FRT5-flanked pSV40-NeoR-pA cassette.

C57BL/6J Wild-type from the colony

When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice. While other Arc null mutations are published with behavioral/neurological defects and embryonic lethality, the donating investigator reports that ArcCreER homozygotes are viable and fertile with no behavioral/neurological defects were grossly observed. More detailed behavioral/physiological characterization of homozygous mice has not been evaluated to date.

Wild-type x Heterozygote; Heterozygote x Wild-type