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B6;129-Fzd5tm2Nat/J
Cat. No.: CEMM-07250123
Common Name: Fz5 CKO-AP
This is a conditional allele of the frizzled 5 gene in which the coding region was flanked by loxP sites and an alkaline phosphatase (AP) reporter was placed downstream. Cre-mediated recombination eliminates the coding region in a tissue-specific manner, ablates expression of the gene, and causes alkaline phosphatase (AP) to be expressed under the control of the frizzled 5 promoter. If recombination is mediated by an embryonic tissue-specific Cre, such as Sox2-Cre (e.g.), mice show a loss of cells in the parafasicular nucleus of the thalamus and defects in eye development.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Fzd5tm2Nat
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Conditional ready (e.g. floxed); No functional change
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| Gene Symbol |
Fzd5
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| Gene Name |
frizzled class receptor 5
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| Chromosome |
1
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| MGI Accession ID | show more close |
| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
A loxP site was inserted downstream of exon 1 and a cassette containg a 5' loxP site, alkaline phosphatase gene and an FRT flanked neo gene was inserted downstream of exon 2. Germ line, flp-mediated recombination was used to remove the neo cassette leaving exon 2 floxed and the alkaline phosphatase gene intact. This is a floxed allele and a reporter allele.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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