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B6;129-Dlg4tm1.1Hnz/J

Cat. No.: CEMM-07250587

Common Name: PSD-95-ENABLED
Cre-mediated excision of a floxed exon 19-20/neomycin stop cassette enables expression of functional mVenus-labeled PSD95 (DLG4) synaptic protein without overexpression in an in vivo setting.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Dlg4tm1.1Hnz
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Allele Name
targeted mutation 1
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Allele Attributes
Conditional ready (e.g. floxed); Reporter
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Gene Symbol
Dlg4
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Gene Name
discs large MAGUK scaffold protein 4
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Chromosome
11
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Expressed Genes
YFP, Yellow Fluorescent Protein, jellyfish
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MGI Accession ID
5588003
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Site of Expression
When crossed with germline Hprt-Cre animals to excise the neomycin stop cassette, strong mVenus fluorescence is expressed throughout many brain regions, including hippocampus, neocortex, striatum and thalamus.
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Strain of Origin
(129S6/SvEvTac x C57BL/6J)F1
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Molecular Note
The last two exons of the gene (exons 19 and 20), including the 3' UTR, were duplicated and inserted downstream of the polyadenylation signal and a neomycin selection cassette flanked by FRT sites. Two loxP sites were inserted to flank the native exons 19 and 20 as well as a neomycin stop cassette. The sequence for mVenus was fused in frame to the end of the coding sequence in exon 20. The FRT flanked neomycin cassette was excised through crosses with a germline flp strain.
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HUSBANDRY
Suggested Controls
B6129SF2/J
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Breeding Considerations
Heterozygotes and homozygotes are viable and fertile.
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Breeding Strategy
Wild-type x Heterozygote; Heterozygote x Wild-type
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For Research Use Only.
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