B6(Cg)-Lhx6^{tm1(cre/ERT2)Zjh}/J

Lhx6^{tm1(cre/ERT2)Zjh}

targeted mutation 1

Recombinase-expressing; Inducible

Lhx6

LIM homeobox protein 6

2

cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene

4365737

Tamoxifen-inducible Cre recombinase activity is observed in the cortex of the brain.

C57BL/6

A targeting vector was designed to insert a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an FRT flanked neo cassette into the initiation codon of the Lhx6 (LIM homeobox protein 6) gene. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette. These Lhx6-CreERT2 mice were subsequently bred with wildtype siblings and/or wildtype C57BL/6 mice for at least one generation (and the FLPe transgene was removed)

C57BL/6J Wild-type from the colony

When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J mice.