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STOCK Gt(ROSA)26Sortm1(CAG-EGFP)Fsh/Mmjax
Cat. No.: CEMM-07250186
These RCE:dual mice harbor the R26R CAG-boosted EGFP reporter allele with both a loxP-flanked STOP cassette and FRT-flanked STOP cassette upstream of the EGFP gene. After removal of the flanked STOP cassettes via cre- and Flp-mediated recombination, the EGFP reporter is expressed in cells/tissues where the expression patterns of the individual promoters driving Cre recombinase and FLP recombinase overlap.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-EGFP)Fsh
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
EGFP will be expressed in cells/tissues where promoters driving Cre recombinase and FLP recombinase are expressed.
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| Strain of Origin |
129S6/SvEvTac
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| Molecular Note |
The R26R CAG-boosted EGFP (RCE) reporter targeting vector was designed with (from 5' to 3') the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector), a splice acceptor site, a loxP-flanked transcriptional STOP cassette (PGK-Neo-polyA and 3x-polyA), an FRT flanked modified pBS302 STOP cassette, an enhanced green fluorescent protein (EGFP) sequence, and two AttP-polyA sequences. After removal of the flanked STOP cassettes via Cre- and Flp-mediated recombination, the EGFP reporter is expressed in cells/tissues where the expression patterns of the individual promoters driving Cre recombinase and FLP recombinase overlap.
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| HUSBANDRY | |
|---|---|
| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred together or to wildtype mice from the colony. Homozygous mice may also be bred together.
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For Research Use Only.
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