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B6.Cg-Npr3tm1.1(cre)Hze/J
Cat. No.: CEMM-07250857
Common Name: Npr3-IRES2-Cre-D
Npr3-IRES2-Cre-D knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous natriuretic peptide receptor 3 promoter/enhancer sequences. In the brain, Cre expression is observed to be enriched in cortical layer 5 and in restricted cell populations within the hippocampal formation. These mice may be used to generate conditional mutations for studying gain-or-loss of function and/or fate mapping in NPR3-expressing cells.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Npr3tm1.1(cre)Hze
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| Allele Name |
targeted mutation 1.1
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Npr3
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| Gene Name |
natriuretic peptide receptor 3
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| Chromosome |
15
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Cre expression is observed to be enriched in cortical layer 5 and in restricted cell populations within the hippocampal formation.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The targeting vector contained, from 5' to 3', a partial Npr3 sequence spanning intron 6 up to and including the endogenous stop codon in exon 8 (also with a frt3 site in intron 7), an internal ribosome entry site 2 sequence (IRES2; allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette, a frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequences (PGK/gb2-Neo-pA::frt5::RNA splice acceptor::3'hygro-pA) and replace it with the recombined AttB/AttP site (AttL).
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. it has not been attempted to make this strain homozygous.
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| Breeding Strategy |
Wild-type x Heterozygote; Heterozygote x Wild-type
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For Research Use Only.
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