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B6.Cg-Hpseem1(cre)Ngai/TasicJ
Cat. No.: CEMM-07251102
Common Name: Hpse-P2A-Cre
Hpse-P2A-Cre knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous heparanase promoter/enhancer sequences. These mice may be used to generate conditional mutations for studying function of Hpse-expressing cells.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Hpseem1(cre)Ngai
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| Allele Name |
endonuclease-mediated mutation 1
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| Allele Attributes |
Recombinase-expressing; Epitope tag
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| Gene Symbol |
Hpse
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| Gene Name |
heparanase
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| Chromosome |
5
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| MGI Accession ID | show more close |
| Strain of Origin |
(C57BL/6 x 129S4/SvJae)F1
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| Molecular Note |
Using CRISPR/Cas9 technology, a P2A-Cre (encoding a viral 2A oligopeptide that mediates ribosomal skipping and a codon-optimized Cre gene) and a myc-tag were inserted into the targetted STOP codon of the Heparanase gene (Hpse) on chromosome 9 in embryonic stem cells. Chimeric mice were generated by aggregating ES cells with morula-stage embryos and bred for germline transmission.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. it has not been attempted to make this strain homozygous.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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