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B6.Cg-H2-K1em1Tyj/AjgrJ

Cat. No.: CEMM-07251126

Common Name: H2-K1Strep
KbStrep knock-in mice have an inverted StrepTagII, flanked by two pairs of inward-facing heterospecific Lox sites, inserted into intron 1 of the H2-K1 gene. Upon cre induction, recombination of the Lox sites results in the inversion of the StrepTagII into the correct transcriptional orientation. These mice may be useful for the selective purification of MHC-I peptide antigens from cell type(s) of interest from intact tissues.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
H2-K1em1Tyj
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Allele Name
endonuclease-mediated mutation 1
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Allele Attributes
Conditional ready (e.g. floxed)
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Gene Symbol
H2-K1
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Gene Name
histocompatibility 2
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Chromosome
17
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MGI Accession ID
7382781
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Strain of Origin
(B6.129P2-Trp53tm1Brn/J x B6(129X1)-Dstem13Dcr/DcrJ)F1
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Molecular Note
CRISPR/Cas9 genome editing technology is used to insert a Cre-inducible affinity tag, StrepTagII, into intron 1 of the gene. The vector included two pairs of inward-facing heterospecific Lox sites (loxP and lox2272) flanking an inverted StrepTagII.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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