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B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J

Cat. No.: CEMM-07250078

Common Name: Ai9; Ai9(RCL-tdT)
Genetic Background: C57BL/6J
Ai9 is a Cre reporter tool strain designed to have a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. Ai9 mice express robust tdTomato fluorescence following Cre-mediated recombination. This strain is congenic on the C57BL/6J genetic background. The Ai9 allele is very similar in design to the Ai14 allele - differing only in the presence (Ai9) or absence (Ai14) of an att site-flanked neo selection cassette at the 3' end of the targeted allele. Importantly, both Ai9 and Ai14 may exhibit low levels of tdTomato expression prior to exposure to Cre recombinase - but the tdTomato expression levels after Cre recombination are greater than those baseline levels. As such, it is recommended that researchers include Cre-negative controls to establish the baseline tdTomato levels in their experiments. See Detailed Description for additional details.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Gt(ROSA)26Sortm9(CAG-tdTomato)Hze
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Allele Name
targeted mutation 9
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Allele Attributes
Conditional ready (e.g. floxed); Reporter
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Gene Symbol
Gt(ROSA)26Sor
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Gene Name
gene trap ROSA 26
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Chromosome
6
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Expressed Genes
RFP, Red Fluorescent Protein, coral
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MGI Accession ID
3809523
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Site of Expression
A loxP-flanked STOP cassette is designed to prevent transcription of the red fluorescent protein variant tdTomato. Upon exposure to Cre recombinase, robust tdTomato fluorescence may be observed. Importantly, the donating investigator reports that very low levels of tdTomato expression may be present prior to introduction of Cre recombinase - but the tdTomato expression levels after Cre recombination are significantly greater than those baseline levels.
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Strain of Origin
(129S6/SvEvTac x C57BL/6NCrl)F1
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Molecular Note
The Rosa-CAG-LSL-tdTomato-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), tdTomato sequence (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
Mutant mice were bred to C57BL/6J inbred mice for many generations to establish this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
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Breeding Strategy
Homozygote x Homozygote
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Coat Color
The coat color is black.
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For Research Use Only.
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