B6.Cg-Gt(ROSA)26Sor^{tm1(CAG-mNeonGreen/mRuby2)Mnz}/J

Gt(ROSA)26Sor^{tm1(CAG-mNeonGreen/mRuby2)Mnz}

targeted mutation 1

Conditional ready (e.g. floxed); Reporter

Gt(ROSA)26Sor

gene trap ROSA 26

6

RFP, Red Fluorescent Protein, coral; GFP, Green Fluorescent Protein

6156806

Cre-mediated excision of a loxP-flanked stop cassette enables tissue-specific expression of the INDIA apoptosis reporter in these Rosa26LSL-INDIA mice.

B6(Cg)-Tyrc-2J

A CAG promoter, loxP-flanked STOP/neomycin cassette in reverse transcriptional orientation, a FRET-based INDIcator of Apoptosis (INDIA) cassette, a single FRT site (remaining after Flp-expressing bacteria were used to excise an FRT flanked IRES-EGFP cassette from the targeting vector), and polyA were introduced to intron 1. The INDIA reporter consists of an optimized fluorescence (Forster) resonance energy transfer (FRET) pair: N-terminally Myc-tagged mNeonGreen and mRuby2 linked by an 18 amino acid peptide containing an aCasp3-cleavage site (DEVDG). When aCasp3 (Casp3, caspase 3; a marker for apoptosis) cleaves the linker, mNeonGreen and mRuby2 are separated, resulting in FRET loss (FRET) and inCreased emission from mNeonGreen. Non-apoptotic cells retaining uncleaved mNeonGreen/mRuby2 (FRET) demonstrate more yellow fluorescence indicating a lower ratio of mNeonGreen/FRET fluorescence emission.

C57BL/6J

Heterozygotes and homozygotes are viable and fertile.

Heterozygote x Heterozygote