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NLS-Cre Recombinase Self-Amplifying mRNA, SA-NLS-Cre
Cat. No.: CEPM-05232155
Product Description: The self-amplifying NLS-Cre mRNA (SA-NLS-Cre) encodes four nonstructural proteins (NSP1 to NSP4) derived from a positive-stranded alphavirus venezuelan equine encephalitis virus (VEEV), and human codon-optimized Cre recombinase fused to nuclear localization sequence. Upon entering the cytoplasm, the mRNA is translated to produce replicase complex, which amplifies the original NLS-Cre mRNA template to produce numerous copies of itself (mRNA replication), thus driving robust NLS-Cre protein expression. It can be used for loxP recombination in cell lines and primary cells.
Codon Optimization: Human & Mouse
| Gene/Insert | |
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| Gene/Insert Name |
NLS-Cre
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| Cloning Information | |
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| 5' Sequencing Primer |
5' Capped with vaccinia capping enzyme system and 2'-O-methyltransferase to yield cap-1 structure
Pseudo-UTP
5mCTP
PolyA: 180 nt
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| Shipping & Handling | |
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| Shipping |
Shipped on dry ice.
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| Storage |
Store at -80 °C.
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| Storage Buffer |
1 mL Double-distilled Water, RNase-free.
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| Quality Control |
Agarose gel electrophoresis. Activity assay: HEK293 cells transfected with LoxP reporter plasmid.
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