Cy5-modified NLS-Flp Recombinase mRNA

Cat. No.: CEPM-05232158

Product Description: This NLS-FLP mRNA will express human codon optimized Flp recombinase fused to nuclear localization sequence. The mRNA can be used for FRT recombination in cell lines and primary cells. The expression of NLS-FLP protein can be visualized by a Cy5 filter.

Codon Optimization: Human

Gene/Insert
Gene/Insert Name
NLS-FLP
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Cloning Information
5' Sequencing Primer
5' Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1) Cyanine Cy5-UTP:N1-Methyl-Pseudouridine-5'-Triphosphate at a ratio of 1:3 5mCTP PolyA: 180 nt
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Shipping & Handling
Shipping
Shipped on dry ice.
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Storage
Store at -80 °C.
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Storage Buffer
1 mL Double-distilled Water, RNase-free.
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Quality Control
Agarose gel electrophoresis. Activity assay: HEK293 cells transfected with FRT reporter plasmid.
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For Research Use Only.
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