Luciferase-2A-Cre (GFP-Bsd) lentiviral particles

Pre-made lentiviral particles bicistronically express two individual proteins: firefly luciferase and nuclear permeable CRE recombinase, under the same suCMV promoter. A GFP-Blasticidin fusion dual marker was expressed under RSV promoter, which allow to select the positive transduced stable cells for long term culture via GFP cell sorting or via antibiotic selection.

Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. Cre lentiviral particles can catalyze the jointing event in Vivo in the presence of lox sites. This nuclear permeant Cre (NLS-Cre) increases the recombination events in vivo. Purified Cre enzyme can joint individual plasmids containing lox sites.

Luciferase and NLS-Cre are constitutively expressed in high level, and optionally, also can be used as tetracycline inducible expression as desired. Luciferase and GFP provide the convenient, realtime monitoring signals.

Suitable for enzymes’ assays in vivo or in vitro

suCMV

GFP

Blasticidin antibiotic selection.

293T cells were transduced with serial dilutions of this product, harvested several days later, and genomic DNA was isolated. Copies of RRE were measured and normalized to RPP30.

Viral particles are shipped frozen on dry ice. Plasmid DNA will also be included in the shipment.

Our lentivirus are stable for at least 1 year if kept at -80 °C, avoid freeze-thaw cycle. Re-freezed unused particles should be kept at 4 °C within 3-5 days.

In DMEM medium with 10% FBS.

Regular lentivirus in DMEM medium.

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2.