Cre (CMV Promoter, Puro) Lentivirus

Pre-made lentivirus express CRE recombinase under inducible suCMV promoter. CRE contains a NLS (nuclear localization signal) at its N-term. This nuclear permanent CRE increases the recombination events in vivo. A Puromycin marker under RSV promoter, allows to select transduced cells for long term expression via blasticidin antibiotic.

Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. Cre lentiviral particles can catalyze the jointing event in Vivo in the presence of lox sites. Purified Cre enzyme can joint individual plasmids containing lox sites.

NLS-Cre is constitutively expressed in high level, and optionally, also can be used as tetracycline inducible expression as desired.

suCMV

Blasticidin antibiotic selection.

293T cells were transduced with serial dilutions of this product, harvested several days later, and genomic DNA was isolated. Copies of RRE were measured and normalized to RPP30.

Viral particles are shipped frozen on dry ice. Plasmid DNA will also be included in the shipment.

Our lentivirus are stable for at least 1 year if kept at -80 °C, avoid freeze-thaw cycle. Re-freezed unused particles should be kept at 4 °C within 3-5 days.

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2.