Cre (RFP-Bsd) (CAG Promoter) Lentivirus

Pre-made lentiviral particles expressing sequence verified CRE recombinase under the CAG promoter which is the strongest promoter in embryonic stem (ES) cells. CRE contains a NLS (nuclear localization signal) at its N-term. This nuclear permeant CRE increases the recombination events in vivo. A RFP-Blasticidin fusion dual marker was expressed under RSV promoter, which allows to select the positive transduced stable cells for long term culture via RFP cell sorting or via antibiotic selection.

Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. Cre lentiviral particles can catalyze the jointing event in Vivo in the presence of lox sites. Purified Cre enzyme can joint individual plasmids containing lox sites.

This product constitutively expresses nuclear localized Cre enzyme, specially high level in embryonic stem (ES) cells.

CAG

Blasticidin antibiotic selection.

293T cells were transduced with serial dilutions of this product, harvested several days later, and genomic DNA was isolated. Copies of RRE were measured and normalized to RPP30.

Viral particles are shipped frozen on dry ice. Plasmid DNA will also be included in the shipment.

Our lentivirus are stable for at least 1 year if kept at -80 °C, avoid freeze-thaw cycle. Re-freezed unused particles should be kept at 4 °C within 3-5 days.

In DMEM medium with 10% FBS and 10x polybrene.

Regular lentivirus in DMEM medium.

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2.