Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre), Endotoxin-free

Recombinant Cre recombinase (TAT-Cre) was purified from an HEK293 cell line expressing enhanced form of Cre Recombinase from bacteriophage P1. This Cre recombinase has an N-terminal 6XHis tag, a Tat peptide (GRKKRRQRRRPPAGTSVSL) and an NLS sequence (PKKKRKV). Tat-Cre is the most effective protein in transduction (in vivo) and subsequent recombination compared to other forms of Cre recombinases, e.g., HNC, TCH6, HC, HNCM, CH.

HEK293T- Cre reporter cell line 80~100% recombination efficiencies.

HEK 293 cells

< 0.1 EU/μg

A standard of 100 Units is defined as the amount of Tat-Cre in 1.0 mL of tissue culture medium that is required to induce 90% GFP expression in a HEK 293T loxP reporter cell line assay.

2 years at -80 °C. 6 months at -20 °C. Upto 3 weeks at 4 °C

In vitro LoxP recombination for subcloning or vector/clone engineering Transduction into cultured cells including stem cells ex vivo

-20 °C ~ -80 °C

Shipped on dry ice.