Cre Recombinase, HNC

Recombinant Cre recombinase HNCre was purified from an E. coli strain carrying an engineered plasmid encoding Cre Recombinase from bacteriophage P1 with additional N-terminal 6XHis tag and an NLS sequence (PKKKRKV). This Cre recombinase has lower cell permeability than Tat-Cre, but can be transported into cells using cell permeable peptides and some protein transfection reagents. It is effective in recombination in various cell types after being transported into cells

40 kDa

< 0.1 EU/μg

> 95% by SDS-PAGE and HPLC analysis.

2 years at -80 °C. 6 months at -20 °C. Upto 3 weeks at 4 °C

In vitro LoxP recombination for subcloning or vector/clone engineering Transduction into cultured cells including stem cells ex vivo

-20 °C ~ -80 °C

Shipped on dry ice.