Cre-2A-GFP(CMV promoter), Concentrated Lentivirus

Cat. No.: CEVP-00450045 Product Size: 1 vial Inquiry
Product Overview
Description
Concentrated Lentivirus bicistronically express two individual proteins: nuclear permeable CRE recombinase and the GFP marker under the same suCMV promoter. It does not contain any antibiotic selection marker.
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Protein Information
Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. Cre lentiviral particles can catalyze the jointing event in Vivo in the presence of lox sites. Purified Cre enzyme can joint individual plasmids containing lox sites.
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Applications
Suitable for hard-to-transduced cell line, or serum-free cell culture, or directly used for in vivo application.
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Promoter Types
suCMV
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Antibiotic Selection
Does not contain any antibiotic selection marker.
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Quality Control
293T cells were transduced with serial dilutions of this product, harvested several days later, and genomic DNA was isolated. Copies of RRE were measured and normalized to RPP30.
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Shipping & Handling
Shipping
Viral particles are shipped frozen on dry ice. Plasmid DNA will also be included in the shipment.
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Storage
Our lentivirus are stable for at least 1 year if kept at -80 °C, avoid freeze-thaw cycle. Re-freezed unused particles should be kept at 4 °C within 3-5 days.
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Storage Buffer
In PBS.
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Formats
Concentrated and resuspended in PBS solution.
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Biosafety Level
Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2.
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For Research Use Only.
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