Cre-2A-GFP (CMV, Neo), Concentrated Lentivirus

Pre-made lentiviral particles bicistronically express two individual proteins: nuclear permeable CRE recombinase and the GFP marker under the same suCMV promoter. A Neomycin marker was expressed under RSV promoter. Particles allows to sort or select transduced cells for long term expression via GFP signal or via Neomycin antibiotic selection.

Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. Cre lentiviral particles can catalyze the jointing event in Vivo in the presence of lox sites. Purified Cre enzyme can joint individual plasmids containing lox sites.

Cre and GFP are constitutively expressed in high level, and optionally, also can be used as tetracycline inducible expression as desired. GFP signal provides a convenient, realtime monitoring the particles performance.

Suitable for hard-to-transduced cell lines, or serum-sensitive cell lines and in vivo application.

suCMV

GFP

Neomycin antibiotic selection.

293T cells were transduced with serial dilutions of this product, harvested several days later, and genomic DNA was isolated. Copies of RRE were measured and normalized to RPP30.

Viral particles are shipped frozen on dry ice. Plasmid DNA will also be included in the shipment.

Our lentivirus are stable for at least 1 year if kept at -80 °C, avoid freeze-thaw cycle. Re-freezed unused particles should be kept at 4 °C within 3-5 days.

In PBS.

Concentrated and resuspended in PBS solution.

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2.