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B6.Cg-Igs7tm224(CAG-EGFP, CAG-dTomato)Tasic/J
Cat. No.: CEMM-07251110
Common Name: TICL-nls-EGFP-ICF-nls-dTom
Ai224 (also called Ai224 (TICL-nls-EGFP-ICF-nls-dTom)) is a dual-recombinase responsive reporter allele designed with a CAG promoter, loxP-STOP-loxP and EGFP coding sequence with a nuclear localization sequence followed by a CAG promoter, frt-STOP-frt and dTomato coding sequence with a nuclear localization sequence - all inserted into the Igs7 locus (TIGRE). Exposure to either Cre or FLP recombinase results in nuclear localized EGFP fluorescence or nuclear localized dTomato fluorescence, respectively. Furthermore, Ai224 are a Cre::FLP intersectional fluorescent reporter line as they are designed to allow both nuclear localized EGFP and nuclear localized dTomato fluorescence in those cells where Cre and FLP expression overlaps.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs7tm224(CAG-EGFP,CAG-dTomato)Tasic
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| Allele Name |
targeted mutation 224
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Igs7
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| Gene Name |
intergenic site 7
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| Chromosome |
9
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| MGI Accession ID | show more close |
| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The targeting vector contains from (from 5' to 3') a Bxb1 attB site, a partial green fluorescent protein sequence (non-functional 500bp fragment to inCrease the space between the Bxb1 attB site and the insulator sequence to improve stability of the targeting vector), two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from pCAGGS), a loxP-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), a synthetic enhanced green fluorescent protein sequence (EGFP) with a nuclear localization sequence, a BGH polyA, two copies of chicken beta-globin HS4 insulator element, the CAG promoter, an FRT flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-TKpA), a dTomato sequence with a nuclear localization sequence (tdT; encoding the non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein [tandem dimer]), a BGH polyA, a PhiC31 attB site, a PGK-5'hygro cassette, an RNA splice donor, a Bxb1 attR site, SA, 3'hygro, SV40pA, and PhiC31 attP.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Heterozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. To date, it has not been attempted to make this strain homozygous. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice.
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| Breeding Strategy |
Wild-type x Heterozygote; Heterozygote x Wild-type
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For Research Use Only.
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