Online Inquiry
B6;129-Igs1tm9(CAG-Bgeo, -Fzd3)Nat/J
Cat. No.: CEMM-07250515
This knock-in, 2 kb 5' of the Ubb (ubiquitin B) gene, gives constitutive expression of a nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo) in the absence of cre-mediated recombination. Following cre-mediated recombination, the NLS-beta-geo coding region is excised and the downstream triple HA epitope (3HA) tagged Fzd3 (frizzled homolog 3 (Drosophila)) coding region is expressed.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs1tm9(CAG-Bgeo,-Fzd3)Nat
show more
close
|
| Allele Name |
targeted mutation 9
show more
close
|
| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence
show more
close
|
| Gene Symbol |
Igs1
show more
close
|
| Gene Name |
intergenic site 1
show more
close
|
| Chromosome |
11
show more
close
|
| Expressed Genes |
HA tag, HA tag; Fzd3, frizzled class receptor 3, mouse, laboratory
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
The HA tagged Fzd3 can be activated by Cre recombinase in any cell in the body at any time in development following Cre-mediated recombination.
show more
close
|
| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
show more
close
|
| Molecular Note |
The following elements were inserted, in order into a region 2 kb 5' of the Ubb gene by targetedrecombination: CMV enhancer/beta-actin promoter, loxP site, beta-geo, 3 stop transcription sites, loxP site, 3HA-Fzd3, rabbit beta-globin 3' UTR. The 3HA tag is inserted after the signal peptide and before the cysteine-rich domain of Fzd3.
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6129SF2/J
show more
close
|
| Breeding Considerations |
Heterozygotes are viable and fertile, but homozygotes are unhealthy.
show more
close
|
For Research Use Only.
Related Products