Cre reporter HEK293 Cell Line

This cell line is generated from HEK293 cells by transducing a lentivirus that carries a human DNA fragment in which two loxP sites in the same direction are included. When Cre recombinase is present, the genomic DNA flanked by loxP sites is deleted, resulting in a larger DNA fragment amplified by PCR than the wild-type DNA.

Cre Recombinase Reporter HEK293 Cell Line was derived from HEK293 cells by transducing a lentivirus that carries a human genomic DNA fragment that contains an insertion with two loxP sites in the same direction. This cell line will be very useful as a sensitive reporter for detecting Cre recombinase activity. When cells were transfected with a Cre expression plasmid, the insertion was deleted in the stable cell line, but not in non-transgenic cells or wild type cells.

Ideal for detecting Cre recombinase activity.

HEK293

Embryonic Kidney Cells

Human

Neomycin antibiotic selection.

EF1

LoxP-NEO-LoxP

Shipped on dry ice.

In 90% DMEM, 10% FBS, 1% Penn/Strep

Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.

BSL-2