Cre (CMV Promoter, Puro), Concentrated Lentivirus

Cat. No.: CEVP-00450016 Product Size: 1 vial Inquiry

Product Overview
Description	Pre-made lentivirus express CRE recombinase under inducible suCMV promoter. CRE contains a NLS (nuclear localization signal) at its N-term. This nuclear permeant CRE increases the recombination events in vivo. A Puromycin marker under RSV promoter, allows to select transduced cells for long term expression via blasticidin antibiotic. show more close
Protein Information	Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. Cre lentiviral particles can catalyze the jointing event in Vivo in the presence of lox sites. Purified Cre enzyme can joint individual plasmids containing lox sites. show more close
Protein Expression Level	NLS-Cre is constitutively expressed in high level, and optionally, also can be used as tetracycline inducible expression as desired. show more close
Applications	Suitable for hard-to-transduced cell lines, or serum-sensitive cell lines and in vivo application. show more close
Promoter Types	suCMV show more close
Antibiotic Selection	Blasticidin antibiotic selection. show more close
Quality Control	293T cells were transduced with serial dilutions of this product, harvested several days later, and genomic DNA was isolated. Copies of RRE were measured and normalized to RPP30. show more close

Shipping & Handling
Shipping	Viral particles are shipped frozen on dry ice. Plasmid DNA will also be included in the shipment. show more close
Storage	Our lentivirus are stable for at least 1 year if kept at -80 °C, avoid freeze-thaw cycle. Re-freezed unused particles should be kept at 4 °C within 3-5 days. show more close
Storage Buffer	In PBS. show more close
Formats	Concentrated and resuspended in PBS solution. show more close
Biosafety Level	Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. show more close

For Research Use Only.