Cre-2A-RFP (Ef1a Pro, Bsd) Lentivirus

Pre-made lentiviral particles bicistronically express two individual proteins: nuclear permeable CRE recombinase and the RFP marker under the same enhance EF1a promoter. CRE contains a NLS (nuclear localization signal) at its N-term. This nuclear permeant CRE increases the recombination events in vivo. A Blasticidin marker was expressed under RSV promoter. Particles allows to sort or select transduced cells for long term expression via RFP signal or via blasticidin antibiotic selection.

Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. Cre lentiviral particles can catalyze the jointing event in Vivo in the presence of lox sites. Purified Cre enzyme can joint individual plasmids containing lox sites.

Cre and RFP are constitutively expressed in high level independent of cell types. RFP signal provides a convenient, realtime monitoring the particles performance.

Ef1a

RFP

Blasticidin antibiotic selection.

293T cells were transduced with serial dilutions of this product, harvested several days later, and genomic DNA was isolated. Copies of RRE were measured and normalized to RPP30.

Viral particles are shipped frozen on dry ice. Plasmid DNA will also be included in the shipment.

Our lentivirus are stable for at least 1 year if kept at -80 °C, avoid freeze-thaw cycle. Re-freezed unused particles should be kept at 4 °C within 3-5 days.

In DMEM medium with 10% FBS and 10x polybrene.

Regular lentivirus in DMEM medium.

Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2.