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Codon-optimized Cre Recombinase, IRES-RFP, Non-Integrative Lentivirus
Cat. No.: CEVP-00450005
Product Size: 1 vial
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Product Overview | |
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Description |
Codon-optimized (humanized) Cre Recombinase, with IRES-RFP, non-integrative lentivirus
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Protein Information |
Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. Cre lentiviral particles can catalyze the jointing event in Vivo in the presence of lox sites. Purified Cre enzyme can joint individual plasmids containing lox sites.
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Applications |
Humanized Cre Recombinase with NLS sequence (PKKKRKV) and IRES-RFP, as a form of non-integrative lentivirus, can be used to induce LoxP recombination in organs (in vivo), or in cultured dividing or non-dividing cells, including stem cells and primary cells.
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Promoter Types |
suCMV
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Fluorescent Marker |
RFP
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Quality Control |
293T cells were transduced with serial dilutions of this product, harvested several days later, and genomic DNA was isolated. Copies of RRE were measured and normalized to RPP30.
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Shipping & Handling | |
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Shipping |
Viral particles are shipped frozen on dry ice. Plasmid DNA will also be included in the shipment.
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Storage |
Our lentivirus are stable for at least 1 year if kept at -80 °C, avoid freeze-thaw cycle. Re-freezed unused particles should be kept at 4 °C within 3-5 days.
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Formats |
Sterilized in PBS
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Biosafety Level |
Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2.
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For Research Use Only.
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