Codon-optimized Cre Recombinase, IRES-RFP, Non-Integrative Lentivirus

Cat. No.: CEVP-00450005 Product Size: 1 vial Inquiry
Product Overview
Description
Codon-optimized (humanized) Cre Recombinase, with IRES-RFP, non-integrative lentivirus
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Protein Information
Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences, named lox sites. Cre lentiviral particles can catalyze the jointing event in Vivo in the presence of lox sites. Purified Cre enzyme can joint individual plasmids containing lox sites.
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Applications
Humanized Cre Recombinase with NLS sequence (PKKKRKV) and IRES-RFP, as a form of non-integrative lentivirus, can be used to induce LoxP recombination in organs (in vivo), or in cultured dividing or non-dividing cells, including stem cells and primary cells.
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Promoter Types
suCMV
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Fluorescent Marker
RFP
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Quality Control
293T cells were transduced with serial dilutions of this product, harvested several days later, and genomic DNA was isolated. Copies of RRE were measured and normalized to RPP30.
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Shipping & Handling
Shipping
Viral particles are shipped frozen on dry ice. Plasmid DNA will also be included in the shipment.
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Storage
Our lentivirus are stable for at least 1 year if kept at -80 °C, avoid freeze-thaw cycle. Re-freezed unused particles should be kept at 4 °C within 3-5 days.
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Formats
Sterilized in PBS
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Biosafety Level
Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2.
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For Research Use Only.
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