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B6J.Cg-Viptm1(cre)Zjh/AreckJ
Cat. No.: CEMM-07250883
Common Name: Vip-IRES-cre (C57BL/6J); B6J.Vip-IRES-cre
Vip-IRES-Cre mice have Cre recombinase expression directed to Vip-expressing cells by the endogenous promoter/enhancer elements of the vasoactive intestinal polypeptide locus. Cre activity is detected in the neocortex, hippocampus, olfactory bulb, suprachiasmatic nuclei, and other discrete midbrain and brainstem regions. These mice may be useful to study neural GABAergic circuits throughout the mammalian brain. While Vip-IRES-Cre was designed to retain endogenous Vip expression, a 2019 publication indicates this allele has reduced Vip expression - see details below. As such, researchers may use homozygous mice for routine colony maintenance, but should consider using heterozygous Vip-IRES-Cre mice and wildtype littermate controls in all their studies. is a C57BL/6J-congenic Vip-IRES-Cre knock-in mouse line, called Vip-IRES-Cre (C57BL/6J) or B6J.Vip-IRES-Cre.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Viptm1(cre)Zjh
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Vip
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| Gene Name |
vasoactive intestinal polypeptide
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| Chromosome |
10
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Cre recombinase activity is observed in some GABAergic interneurons.
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| Strain of Origin |
(C57BL/6 x 129S4/SvJae)F1
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| Molecular Note |
A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, an SV40 polyA signal, and an FRT flanked neo cassette into the 3' untranslated region (after the translational termination site) of the vasoactive intestinal polypeptide locus (Vip). This construct was electroporated into C57BL/6 x 129S4/SvJae hybrid embryonic stem (ES) cells. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to generate the colony and remove the neo selection cassette. The FLPe transgene has been bred out of the line.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony Repository, homozygous mice may be bred together. While Vip-IRES-cre was designed to retain endogenous Vip expression, a publication indicates this allele has reduced Vip expression - see detailed description. As such, researchers may use homozygous mice for routine colony maintenance, but should consider using heterozygous Vip-IRES-Cre mice and wildtype littermate controls in all their studies.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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